benoit.bruneau@gladstone.ucsf.edu

Benoit Bruneau’s lab is broadly interested in understanding how genes are turned on and off during human development, and how this process is controlled during the formation of the heart in the embryo. Specifically, his team is investigating how errors in this process cause congenital heart disease. They use mouse models and human induced pluripotent stem (iPS) cells to unravel the transcription factor networks that regulate sets of genes critical for heart development.

Disease Areas

Birth Defects

Congenital Heart Disease

Heart Failure

Areas of Expertise

CRISPR and Genome Editing

Disease Models

Gene Regulation

Heart Development

Human Genetics

Stem Cells

Lab Focus

Studying the actions of chromatin remodeling complexes and epigenetic regulators on cardiac genes and their role in heart development and function.

Understanding interactions between disease-related transcription factors and chromatin modifying complexes in the regulation of cardiac morphogenesis.

Investigating the cellular regulation of important morphogenetic processes, such as cardiac septation.

Research Impact

Research in Bruneau’s lab is important for understanding basic concepts in gene regulation and how they are dysregulated in disease. They demonstrated interactions between cardiac transcription factors, which provided new insights into the tight regulation of gene cohorts and has had immediate implications in understanding how mutations in these genes cause similar heart defects. These findings are broadly impactful as they apply to any set of transcription factors, in any cell type. In addition, his team’s work on a protein called CTCF and 3D genome organization resolved several long-standing questions in biology applicable to all cells in the body.

Professional Titles

Director, Gladstone Institute of Cardiovascular Disease

William H. Younger Chair in Cardiovascular Research, Gladstone Institutes

Professor, Department of Pediatrics, UC San Francisco

Bio

Benoit G. Bruneau, PhD, is the director of the Gladstone Institute of Cardiovascular Disease and a senior investigator at Gladstone Institutes. He is also a professor of pediatrics at UC San Francisco.

Originally from Canada, Bruneau earned a bachelor’s degree in biology and a doctorate in physiology at the University of Ottawa. He completed a postdoctoral fellowship in the Department of Genetics at Harvard University Medical School in the lab of Jonathan and Christine Seidman. Before coming to Gladstone, Bruneau led a lab at The Hospital for Sick Children in Toronto and was an assistant professor in the Department of Molecular and Medical Genetics at the University of Toronto.

Holder of the William H. Younger Chair in Cardiovascular Research, he has distinguished himself internationally as a leading figure in the field of epigenetics and gene regulation, particularly as it relates to cardiac biology and disease. He also serves as an editor for the journal Development and sits on the editorial board of Genes & Development.

How Did You Get Your Start In Science?

“My third-year undergraduate developmental biology class had a hands-on Axolotl embryology lab. We did classic fate-map experiments, and beating heart explants. I was hooked.”

Benoit Bruneau, PhD

Honors and Awards

2012 American Heart Association (Fellow)

2010 Established Investigator Award (5 years), Lawrence J. and Florence A. DeGeorge Charitable Trust/American Heart Association

2003 Premier’s Research Excellence Award, Canada

2001 New Investigator Award, Heart and Stroke Foundation of Canada/Canadian Institutes of Health Research

Publications

Salt-inducible kinase 1 maintains HDAC7 stability to promote pathologic cardiac remodeling. Hsu A, Duan Q, McMahon S, Huang Y, Wood SA, Gray NS, Wang B, Bruneau BG, Haldar SM. J Clin Invest. 2020 Feb 27.
Minimal in vivo requirements for developmentally regulated cardiac long intergenic non-coding RNAs. George MR, Duan Q, Nagle A, Kathiriya IS, Huang Y, Rao K, Haldar SM, Bruneau BG. Development. 2019 Dec 09; 146(23).
CTCF confers local nucleosome resiliency after DNA replication and during mitosis. Owens N, Papadopoulou T, Festuccia N, Tachtsidi A, Gonzalez I, Dubois A, Vandormael-Pournin S, Nora EP, Bruneau BG, Cohen-Tannoudji M, Navarro P. Elife. 2019 10 10; 8.
Chromatin and epigenetics in development: a Special Issue. Bruneau BG, Koseki H, Strome S, Torres-Padilla ME. Development. 2019 Oct 10; 146(19).
RNA Interactions Are Essential for CTCF-Mediated Genome Organization. Saldaña-Meyer R, Rodriguez-Hernaez J, Escobar T, Nishana M, Jácome-López K, Nora EP, Bruneau BG, Tsirigos A, Furlan-Magaril M, Skok J, Reinberg D. Mol Cell. 2019 11 07; 76(3):412-422.e5.
Genome of the Komodo dragon reveals adaptations in the cardiovascular and chemosensory systems of monitor lizards. Lind AL, Lai YYY, Mostovoy Y, Holloway AK, Iannucci A, Mak ACY, Fondi M, Orlandini V, Eckalbar WL, Milan M, Rovatsos M, Kichigin IG, Makunin AI, Johnson Pokorná M, Altmanová M, Trifonov VA, Schijlen E, Kratochvíl L, Fani R, Velenský P, Rehák I, Patarnello T, Jessop TS, Hicks JW, Ryder OA, Mendelson JR, Ciofi C, Kwok PY, Pollard KS, Bruneau BG. Nat Ecol Evol. 2019 08; 3(8):1241-1252.
Dynamic BAF chromatin remodeling complex subunit inclusion promotes temporally distinct gene expression programs in cardiogenesis. Hota SK, Johnson JR, Verschueren E, Thomas R, Blotnick AM, Zhu Y, Sun X, Pennacchio LA, Krogan NJ, Bruneau BG. Development. 2019 Jul 05; 146(19).
A De Novo Shape Motif Discovery Algorithm Reveals Preferences of Transcription Factors for DNA Shape Beyond Sequence Motifs. Samee MAH, Bruneau BG, Pollard KS. Cell Syst. 2019 01 23; 8(1):27-42.e6.
Identifying cis Elements for Spatiotemporal Control of Mammalian DNA Replication. Sima J, Chakraborty A, Dileep V, Michalski M, Klein KN, Holcomb NP, Turner JL, Paulsen MT, Rivera-Mulia JC, Trevilla-Garcia C, Bartlett DA, Zhao PA, Washburn BK, Nora EP, Kraft K, Mundlos S, Bruneau BG, Ljungman M, Fraser P, Ay F, Gilbert DM. Cell. 2019 02 07; 176(4):816-830.e18.
Heart enhancers with deeply conserved regulatory activity are established early in zebrafish development. Yuan X, Song M, Devine P, Bruneau BG, Scott IC, Wilson MD. Nat Commun. 2018 11 26; 9(1):4977.
Chromatin Domains Go on Repeat in Disease. Bruneau BG, Nora EP. Cell. 2018 09 20; 175(1):38-40.
A novel reporter allele for monitoring Dll4 expression within the embryonic and adult mouse. Herman AM, Rhyner AM, Devine WP, Marrelli SP, Bruneau BG, Wythe JD. Biol Open. 2018 Mar 12; 7(3).
Cardiac-enriched BAF chromatin-remodeling complex subunit Baf60c regulates gene expression programs essential for heart development and function. Sun X, Hota SK, Zhou YQ, Novak S, Miguel-Perez D, Christodoulou D, Seidman CE, Seidman JG, Gregorio CC, Henkelman RM, Rossant J, Bruneau BG. Biol Open. 2018 Jan 05; 7(1).
Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG. Cell. 2017 May 18; 169(5):930-944.e22.
Cooperative activation of cardiac transcription through myocardin bridging of paired MEF2 sites. Anderson CM, Hu J, Thomas R, Gainous TB, Celona B, Sinha T, Dickel DE, Heidt AB, Xu SM, Bruneau BG, Pollard KS, Pennacchio LA, Black BL. Development. 2017 04 01; 144(7):1235-1241.
Single-Cell Resolution of Temporal Gene Expression during Heart Development. DeLaughter DM, Bick AG, Wakimoto H, McKean D, Gorham JM, Kathiriya IS, Hinson JT, Homsy J, Gray J, Pu W, Bruneau BG, Seidman JG, Seidman CE. Dev Cell. 2016 11 21; 39(4):480-490.
ATP-dependent chromatin remodeling during mammalian development. Hota SK, Bruneau BG. Development. 2016 08 15; 143(16):2882-97.
Loss of Iroquois homeobox transcription factors 3 and 5 in osteoblasts disrupts cranial mineralization. Cain CJ, Gaborit N, Lwin W, Barruet E, Ho S, Bonnard C, Hamamy H, Shboul M, Reversade B, Kayserili H, Bruneau BG, Hsiao EC. Bone Rep. 2016 Dec; 5:86-95.
Expandable Cardiovascular Progenitor Cells Reprogrammed from Fibroblasts. Zhang Y, Cao N, Huang Y, Spencer CI, Fu JD, Yu C, Liu K, Nie B, Xu T, Li K, Xu S, Bruneau BG, Srivastava D, Ding S. Cell Stem Cell. 2016 Mar 03; 18(3):368-81.
KMT2D regulates specific programs in heart development via histone H3 lysine 4 di-methylation. Ang SY, Uebersohn A, Spencer CI, Huang Y, Lee JE, Ge K, Bruneau BG. Development. 2016 Mar 01; 143(5):810-21.
Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis. Luna-Zurita L, Stirnimann CU, Glatt S, Kaynak BL, Thomas S, Baudin F, Samee MA, He D, Small EM, Mileikovsky M, Nagy A, Holloway AK, Pollard KS, Müller CW, Bruneau BG. Cell. 2016 Feb 25; 164(5):999-1014.
Accelerated Evolution of Enhancer Hotspots in the Mammal Ancestor. Holloway AK, Bruneau BG, Sukonnik T, Rubenstein JL, Pollard KS. Mol Biol Evol. 2016 Apr; 33(4):1008-18.
Polycomb Regulates Mesoderm Cell Fate-Specification in Embryonic Stem Cells through Activation and Repression Mechanisms. Morey L, Santanach A, Blanco E, Aloia L, Nora EP, Bruneau BG, Di Croce L. Cell Stem Cell. 2015 Sep 03; 17(3):300-15.
Brg1 modulates enhancer activation in mesoderm lineage commitment. Alexander JM, Hota SK, He D, Thomas S, Ho L, Pennacchio LA, Bruneau BG. Development. 2015 Apr 15; 142(8):1418-30.
Human disease modeling reveals integrated transcriptional and epigenetic mechanisms of NOTCH1 haploinsufficiency. Theodoris CV, Li M, White MP, Liu L, He D, Pollard KS, Bruneau BG, Srivastava D. Cell. 2015 Mar 12; 160(6):1072-86.
Evolution of lysine acetylation in the RNA polymerase II C-terminal domain. Simonti CN, Pollard KS, Schröder S, He D, Bruneau BG, Ott M, Capra JA. BMC Evol Biol. 2015 Mar 10; 15:35.
Investigating the transcriptional control of cardiovascular development. Kathiriya IS, Nora EP, Bruneau BG. Circ Res. 2015 Feb 13; 116(4):700-14.
Looking inwards: opening a window onto human development. Pourquié O, Bruneau B, Keller G, Smith A. Development. 2015 Jan 01; 142(1):1-2.
Ezh2-mediated repression of a transcriptional pathway upstream of Mmp9 maintains integrity of the developing vasculature. Delgado-Olguín P, Dang LT, He D, Thomas S, Chi L, Sukonnik T, Khyzha N, Dobenecker MW, Fish JE, Bruneau BG. Development. 2014 Dec; 141(23):4610-7.
Early patterning and specification of cardiac progenitors in gastrulating mesoderm. Devine WP, Wythe JD, George M, Koshiba-Takeuchi K, Bruneau BG. Elife. 2014 Oct 08; 3.
Finding a niche for cardiac precursors. Bruneau BG. Elife. 2014 May 06; 3:e02993.
HDAC-regulated myomiRs control BAF60 variant exchange and direct the functional phenotype of fibro-adipogenic progenitors in dystrophic muscles. Saccone V, Consalvi S, Giordani L, Mozzetta C, Barozzi I, Sandoná M, Ryan T, Rojas-Muñoz A, Madaro L, Fasanaro P, Borsellino G, De Bardi M, Frigè G, Termanini A, Sun X, Rossant J, Bruneau BG, Mercola M, Minucci S, Puri PL. Genes Dev. 2014 Apr 15; 28(8):841-57.
Function-based identification of mammalian enhancers using site-specific integration. Dickel DE, Zhu Y, Nord AS, Wylie JN, Akiyama JA, Afzal V, Plajzer-Frick I, Kirkpatrick A, Göttgens B, Bruneau BG, Visel A, Pennacchio LA. Nat Methods. 2014 May; 11(5):566-71.
Congenital heart disease: entering a new era of human genetics. Bruneau BG, Srivastava D. Circ Res. 2014 Feb 14; 114(4):598-9.
Acetylation of RNA polymerase II regulates growth-factor-induced gene transcription in mammalian cells. Schröder S, Herker E, Itzen F, He D, Thomas S, Gilchrist DA, Kaehlcke K, Cho S, Pollard KS, Capra JA, Schnölzer M, Cole PA, Geyer M, Bruneau BG, Adelman K, Ott M. Mol Cell. 2013 Nov 07; 52(3):314-24.
An interview with Benoit Bruneau. Bruneau B. Development. 2013 Nov; 140(22):4463-4.
Chromatin modulators as facilitating factors in cellular reprogramming. Luna-Zurita L, Bruneau BG. Curr Opin Genet Dev. 2013 Oct; 23(5):556-61.
Direct reprogramming of human fibroblasts toward a cardiomyocyte-like state. Fu JD, Stone NR, Liu L, Spencer CI, Qian L, Hayashi Y, Delgado-Olguin P, Ding S, Bruneau BG, Srivastava D. Stem Cell Reports. 2013; 1(3):235-47.
ENU-induced mutation in the DNA-binding domain of KLF3 reveals important roles for KLF3 in cardiovascular development and function in mice. Kelsey L, Flenniken AM, Qu D, Funnell AP, Pearson R, Zhou YQ, Voronina I, Berberovic Z, Wood G, Newbigging S, Weiss ES, Wong M, Quach I, Yeh SY, Deshwar AR, Scott IC, McKerlie C, Henkelman M, Backx P, Simpson J, Osborne L, Rossant J, Crossley M, Bruneau B, Adamson SL. PLoS Genet. 2013; 9(7):e1003612.
ETS factors regulate Vegf-dependent arterial specification. Wythe JD, Dang LT, Devine WP, Boudreau E, Artap ST, He D, Schachterle W, Stainier DY, Oettgen P, Black BL, Bruneau BG, Fish JE. Dev Cell. 2013 Jul 15; 26(1):45-58.
Stem cells and regeneration: a special issue. Pourquié O, Bruneau B, Götz M, Keller G, Smith A. Development. 2013 Jun; 140(12):2445.
The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage. Shaffer HB, Minx P, Warren DE, Shedlock AM, Thomson RC, Valenzuela N, Abramyan J, Amemiya CT, Badenhorst D, Biggar KK, Borchert GM, Botka CW, Bowden RM, Braun EL, Bronikowski AM, Bruneau BG, Buck LT, Capel B, Castoe TA, Czerwinski M, Delehaunty KD, Edwards SV, Fronick CC, Fujita MK, Fulton L, Graves TA, Green RE, Haerty W, Hariharan R, Hernandez O, Hillier LW, Holloway AK, Janes D, Janzen FJ, Kandoth C, Kong L, de Koning AP, Li Y, Literman R, McGaugh SE, Mork L, O'Laughlin M, Paitz RT, Pollock DD, Ponting CP, Radhakrishnan S, Raney BJ, Richman JM, St John J, Schwartz T, Sethuraman A, Spinks PQ, Storey KB, Thane N, Vinar T, Zimmerman LM, Warren WC, Mardis ER, Wilson RK. Genome Biol. 2013 Mar 28; 14(3):R28.
Signaling and transcriptional networks in heart development and regeneration. Bruneau BG. Cold Spring Harb Perspect Biol. 2013 Mar 01; 5(3):a008292.
Regulation of retinal interneuron subtype identity by the Iroquois homeobox gene Irx6. Star EN, Zhu M, Shi Z, Liu H, Pashmforoush M, Sauve Y, Bruneau BG, Chow RL. Development. 2012 Dec; 139(24):4644-55.
Cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis and postnatal physiology. Gaborit N, Sakuma R, Wylie JN, Kim KH, Zhang SS, Hui CC, Bruneau BG. Development. 2012 Nov; 139(21):4007-19.
Dynamic and coordinated epigenetic regulation of developmental transitions in the cardiac lineage. Wamstad JA, Alexander JM, Truty RM, Shrikumar A, Li F, Eilertson KE, Ding H, Wylie JN, Pico AR, Capra JA, Erwin G, Kattman SJ, Keller GM, Srivastava D, Levine SS, Pollard KS, Holloway AK, Boyer LA, Bruneau BG. Cell. 2012 Sep 28; 151(1):206-20.
Iroquois homeodomain transcription factors in heart development and function. Kim KH, Rosen A, Bruneau BG, Hui CC, Backx PH. Circ Res. 2012 May 25; 110(11):1513-24.
Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis. Delgado-Olguín P, Huang Y, Li X, Christodoulou D, Seidman CE, Seidman JG, Tarakhovsky A, Bruneau BG. Nat Genet. 2012 Jan 22; 44(3):343-7.
Epigenetics and cardiovascular development. Chang CP, Bruneau BG. Annu Rev Physiol. 2012; 74:41-68.
Iroquois homeobox gene 3 establishes fast conduction in the cardiac His-Purkinje network. Zhang SS, Kim KH, Rosen A, Smyth JW, Sakuma R, Delgado-Olguín P, Davis M, Chi NC, Puviindran V, Gaborit N, Sukonnik T, Wylie JN, Brand-Arzamendi K, Farman GP, Kim J, Rose RA, Marsden PA, Zhu Y, Zhou YQ, Miquerol L, Henkelman RM, Stainier DY, Shaw RM, Hui CC, Bruneau BG, Backx PH. Proc Natl Acad Sci U S A. 2011 Aug 16; 108(33):13576-81.
Atrial natriuretic factor in the developing heart: a signpost for cardiac morphogenesis. Bruneau BG. Can J Physiol Pharmacol. 2011 Aug; 89(8):533-7.
Ezh2 regulates anteroposterior axis specification and proximodistal axis elongation in the developing limb. Wyngaarden LA, Delgado-Olguin P, Su IH, Bruneau BG, Hopyan S. Development. 2011 Sep; 138(17):3759-67.
Tinman/Nkx2-5 acts via miR-1 and upstream of Cdc42 to regulate heart function across species. Qian L, Wythe JD, Liu J, Cartry J, Vogler G, Mohapatra B, Otway RT, Huang Y, King IN, Maillet M, Zheng Y, Crawley T, Taghli-Lamallem O, Semsarian C, Dunwoodie S, Winlaw D, Harvey RP, Fatkin D, Towbin JA, Molkentin JD, Srivastava D, Ocorr K, Bruneau BG, Bodmer R. J Cell Biol. 2011 Jun 27; 193(7):1181-96.
A Slit/miR-218/Robo regulatory loop is required during heart tube formation in zebrafish. Fish JE, Wythe JD, Xiao T, Bruneau BG, Stainier DY, Srivastava D, Woo S. Development. 2011 Apr; 138(7):1409-19.
Chromatin remodelling complex dosage modulates transcription factor function in heart development. Takeuchi JK, Lou X, Alexander JM, Sugizaki H, Delgado-Olguín P, Holloway AK, Mori AD, Wylie JN, Munson C, Zhu Y, Zhou YQ, Yeh RF, Henkelman RM, Harvey RP, Metzger D, Chambon P, Stainier DY, Pollard KS, Scott IC, Bruneau BG. Nat Commun. 2011 Feb 08; 2:187.
CTCF promotes muscle differentiation by modulating the activity of myogenic regulatory factors. Delgado-Olguín P, Brand-Arzamendi K, Scott IC, Jungblut B, Stainier DY, Bruneau BG, Recillas-Targa F. J Biol Chem. 2011 Apr 08; 286(14):12483-94.
An endocardial pathway involving Tbx5, Gata4, and Nos3 required for atrial septum formation. Nadeau M, Georges RO, Laforest B, Yamak A, Lefebvre C, Beauregard J, Paradis P, Bruneau BG, Andelfinger G, Nemer M. Proc Natl Acad Sci U S A. 2010 Nov 09; 107(45):19356-61.
Shox2 mediates Tbx5 activity by regulating Bmp4 in the pacemaker region of the developing heart. Puskaric S, Schmitteckert S, Mori AD, Glaser A, Schneider KU, Bruneau BG, Blaschke RJ, Steinbeisser H, Rappold G. Hum Mol Genet. 2010 Dec 01; 19(23):4625-33.
Chromatin remodeling in heart development. Bruneau BG. Curr Opin Genet Dev. 2010 Oct; 20(5):505-11.
Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Ieda M, Fu JD, Delgado-Olguin P, Vedantham V, Hayashi Y, Bruneau BG, Srivastava D. Cell. 2010 Aug 06; 142(3):375-86.
Epigenetic regulation of the cardiovascular system: introduction to a review series. Bruneau BG. Circ Res. 2010 Aug 06; 107(3):324-6.
Lessons for cardiac regeneration and repair through development. Alexander JM, Bruneau BG. Trends Mol Med. 2010 Sep; 16(9):426-34.
Extensive enteric nervous system abnormalities in mice transgenic for artificial chromosomes containing Parkinson disease-associated alpha-synuclein gene mutations precede central nervous system changes. Kuo YM, Li Z, Jiao Y, Gaborit N, Pani AK, Orrison BM, Bruneau BG, Giasson BI, Smeyne RJ, Gershon MD, Nussbaum RL. Hum Mol Genet. 2010 May 01; 19(9):1633-50.
The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1. Fouladkou F, Lu C, Jiang C, Zhou L, She Y, Walls JR, Kawabe H, Brose N, Henkelman RM, Huang A, Bruneau BG, Rotin D. J Biol Chem. 2010 Feb 26; 285(9):6770-80.
Reptilian heart development and the molecular basis of cardiac chamber evolution. Koshiba-Takeuchi K, Mori AD, Kaynak BL, Cebra-Thomas J, Sukonnik T, Georges RO, Latham S, Beck L, Beck L, Henkelman RM, Black BL, Olson EN, Wade J, Takeuchi JK, Nemer M, Gilbert SF, Bruneau BG. Nature. 2009 Sep 03; 461(7260):95-8.
NKX2-5 regulates the expression of beta-catenin and GATA4 in ventricular myocytes. Riazi AM, Takeuchi JK, Hornberger LK, Zaidi SH, Amini F, Coles J, Bruneau BG, Van Arsdell GS. PLoS One. 2009 May 28; 4(5):e5698.
Directed transdifferentiation of mouse mesoderm to heart tissue by defined factors. Takeuchi JK, Bruneau BG. Nature. 2009 Jun 04; 459(7247):708-11.
Alternative induced pluripotent stem cell characterization criteria for in vitro applications. Ellis J, Bruneau BG, Keller G, Lemischka IR, Nagy A, Rossant J, Srivastava D, Zandstra PW, Stanford WL. Cell Stem Cell. 2009 Mar 06; 4(3):198-9; author reply 202.
miR-126 regulates angiogenic signaling and vascular integrity. Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, Ivey KN, Bruneau BG, Stainier DY, Srivastava D. Dev Cell. 2008 Aug; 15(2):272-84.
Tbx5-dependent pathway regulating diastolic function in congenital heart disease. Zhu Y, Gramolini AO, Walsh MA, Zhou YQ, Slorach C, Friedberg MK, Takeuchi JK, Sun H, Henkelman RM, Backx PH, Redington AN, Maclennan DH, Bruneau BG. Proc Natl Acad Sci U S A. 2008 Apr 08; 105(14):5519-24.
The developmental genetics of congenital heart disease. Bruneau BG. Nature. 2008 Feb 21; 451(7181):943-8.
4D cardiac MRI in the mouse. Feintuch A, Zhu Y, Bishop J, Davidson L, Dazai J, Bruneau BG, Henkelman RM. NMR Biomed. 2007 May; 20(3):360-5.
The heart's Da Vinci code: a Renaissance at Keystone. Bruneau BG, Black BL. Development. 2007 May; 134(9):1631-3.
Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry. Takeuchi JK, Lickert H, Bisgrove BW, Sun X, Yamamoto M, Chawengsaksophak K, Hamada H, Yost HJ, Rossant J, Bruneau BG. Proc Natl Acad Sci U S A. 2007 Jan 16; 104(3):846-51.
Irxl1, a divergent Iroquois homeobox family transcription factor gene. Takeuchi JK, Bruneau BG. Gene Expr Patterns. 2007 Jan; 7(1-2):51-6.
Tbx5-dependent rheostatic control of cardiac gene expression and morphogenesis. Mori AD, Zhu Y, Vahora I, Nieman B, Koshiba-Takeuchi K, Davidson L, Pizard A, Seidman JG, Seidman CE, Chen XJ, Henkelman RM, Bruneau BG. Dev Biol. 2006 Sep 15; 297(2):566-86.
Chromatin modification and remodeling in heart development. Delgado-Olguín P, Takeuchi JK, Bruneau BG. ScientificWorldJournal. 2006 Apr 24; 6:1851-61.
[Irx5: a transcription factor that regulates the cardiac repolarization gradient]. Bruneau BG. Med Sci (Paris). 2006 Mar; 22(3):231-2.
Cooperative and antagonistic interactions between Sall4 and Tbx5 pattern the mouse limb and heart. Koshiba-Takeuchi K, Takeuchi JK, Arruda EP, Kathiriya IS, Mo R, Hui CC, Srivastava D, Bruneau BG. Nat Genet. 2006 Feb; 38(2):175-83.
The homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient. Costantini DL, Arruda EP, Agarwal P, Kim KH, Zhu Y, Zhu W, Lebel M, Cheng CW, Park CY, Pierce SA, Guerchicoff A, Pollevick GD, Chan TY, Kabir MG, Cheng SH, Husain M, Antzelevitch C, Srivastava D, Gross GJ, Hui CC, Backx PH, Bruneau BG. Cell. 2005 Oct 21; 123(2):347-58.
A Gja1 missense mutation in a mouse model of oculodentodigital dysplasia. Flenniken AM, Osborne LR, Anderson N, Ciliberti N, Fleming C, Gittens JE, Gong XQ, Kelsey LB, Lounsbury C, Moreno L, Nieman BJ, Peterson K, Qu D, Roscoe W, Shao Q, Tong D, Veitch GI, Voronina I, Vukobradovic I, Wood GA, Zhu Y, Zirngibl RA, Aubin JE, Bai D, Bruneau BG, Grynpas M, Henderson JE, Henkelman RM, McKerlie C, Sled JG, Stanford WL, Laird DW, Kidder GM, Adamson SL, Rossant J. Development. 2005 Oct; 132(19):4375-86.
The Iroquois homeobox gene, Irx5, is required for retinal cone bipolar cell development. Cheng CW, Chow RL, Lebel M, Sakuma R, Cheung HO, Thanabalasingham V, Zhang X, Bruneau BG, Birch DG, Hui CC, McInnes RR, Cheng SH. Dev Biol. 2005 Nov 01; 287(1):48-60.
Genome-wide analysis of mouse transcripts using exon microarrays and factor graphs. Frey BJ, Mohammad N, Morris QD, Zhang W, Robinson MD, Mnaimneh S, Chang R, Pan Q, Sat E, Rossant J, Bruneau BG, Aubin JE, Blencowe BJ, Hughes TR. Nat Genet. 2005 Sep; 37(9):991-6.
Developmental biology: tiny brakes for a growing heart. Bruneau BG. Nature. 2005 Jul 14; 436(7048):181-2.
Connexin 40, a target of transcription factor Tbx5, patterns wrist, digits, and sternum. Pizard A, Burgon PG, Paul DL, Bruneau BG, Seidman CE, Seidman JG. Mol Cell Biol. 2005 Jun; 25(12):5073-83.
Abnormal cardiac inflow patterns during postnatal development in a mouse model of Holt-Oram syndrome. Zhou YQ, Zhu Y, Bishop J, Davidson L, Henkelman RM, Bruneau BG, Foster FS. Am J Physiol Heart Circ Physiol. 2005 Sep; 289(3):H992-H1001.
Tbx20 dose-dependently regulates transcription factor networks required for mouse heart and motoneuron development. Takeuchi JK, Mileikovskaia M, Koshiba-Takeuchi K, Heidt AB, Mori AD, Arruda EP, Gertsenstein M, Georges R, Davidson L, Mo R, Hui CC, Henkelman RM, Nemer M, Black BL, Nagy A, Bruneau BG. Development. 2005 May; 132(10):2463-74.
Serum response factor, an enriched cardiac mesoderm obligatory factor, is a downstream gene target for Tbx genes. Barron MR, Belaguli NS, Zhang SX, Trinh M, Iyer D, Merlo X, Lough JW, Parmacek MS, Bruneau BG, Schwartz RJ. J Biol Chem. 2005 Mar 25; 280(12):11816-28.
The functional landscape of mouse gene expression. Zhang W, Morris QD, Chang R, Shai O, Bakowski MA, Mitsakakis N, Mohammad N, Robinson MD, Zirngibl R, Somogyi E, Laurin N, Eftekharpour E, Sat E, Grigull J, Pan Q, Peng WT, Krogan N, Greenblatt J, Fehlings M, van der Kooy D, Aubin J, Bruneau BG, Rossant J, Blencowe BJ, Frey BJ, Hughes TR. J Biol. 2004; 3(5):21.
Baf60c is essential for function of BAF chromatin remodelling complexes in heart development. Lickert H, Takeuchi JK, Von Both I, Walls JR, McAuliffe F, Adamson SL, Henkelman RM, Wrana JL, Rossant J, Bruneau BG. Nature. 2004 Nov 04; 432(7013):107-12.
Lats2/Kpm is required for embryonic development, proliferation control and genomic integrity. McPherson JP, Tamblyn L, Elia A, Migon E, Shehabeldin A, Matysiak-Zablocki E, Lemmers B, Salmena L, Hakem A, Fish J, Kassam F, Squire J, Bruneau BG, Hande MP, Hakem R. EMBO J. 2004 Sep 15; 23(18):3677-88.
The T-Box transcription factor Tbx5 is required for the patterning and maturation of the murine cardiac conduction system. Moskowitz IP, Pizard A, Patel VV, Bruneau BG, Kim JB, Kupershmidt S, Roden D, Berul CI, Seidman CE, Seidman JG. Development. 2004 Aug; 131(16):4107-16.
Tbx1 has a dual role in the morphogenesis of the cardiac outflow tract. Xu H, Morishima M, Wylie JN, Schwartz RJ, Bruneau BG, Lindsay EA, Baldini A. Development. 2004 Jul; 131(13):3217-27.
TBX5 mutations and congenital heart disease: Holt-Oram syndrome revealed. Mori AD, Bruneau BG. Curr Opin Cardiol. 2004 May; 19(3):211-5.
The Iroquois homeobox gene Irx2 is not essential for normal development of the heart and midbrain-hindbrain boundary in mice. Lebel M, Agarwal P, Cheng CW, Kabir MG, Chan TY, Thanabalasingham V, Zhang X, Cohen DR, Husain M, Cheng SH, Bruneau BG, Hui CC. Mol Cell Biol. 2003 Nov; 23(22):8216-25.
Cardiac T-box factor Tbx20 directly interacts with Nkx2-5, GATA4, and GATA5 in regulation of gene expression in the developing heart. Stennard FA, Costa MW, Elliott DA, Rankin S, Haast SJ, Lai D, McDonald LP, Niederreither K, Dolle P, Bruneau BG, Zorn AM, Harvey RP. Dev Biol. 2003 Oct 15; 262(2):206-24.
Tbx5 is required for forelimb bud formation and continued outgrowth. Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP. Development. 2003 Jun; 130(12):2741-51.
The developing heart and congenital heart defects: a make or break situation. Bruneau BG. Clin Genet. 2003 Apr; 63(4):252-61.
Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo. Agarwal P, Wylie JN, Galceran J, Arkhitko O, Li C, Deng C, Grosschedl R, Bruneau BG. Development. 2003 Feb; 130(3):623-33.
Transcriptional regulation of vertebrate cardiac morphogenesis. Bruneau BG. Circ Res. 2002 Mar 22; 90(5):509-19.
A murine model of Holt-Oram syndrome defines roles of the T-box transcription factor Tbx5 in cardiogenesis and disease. Bruneau BG, Nemer G, Schmitt JP, Charron F, Robitaille L, Caron S, Conner DA, Gessler M, Nemer M, Seidman CE, Seidman JG. Cell. 2001 Sep 21; 106(6):709-21.
Cardiomyopathy in Irx4-deficient mice is preceded by abnormal ventricular gene expression. Bruneau BG, Bao ZZ, Fatkin D, Xavier-Neto J, Georgakopoulos D, Maguire CT, Berul CI, Kass DA, Kuroski-de Bold ML, de Bold AJ, Conner DA, Rosenthal N, Cepko CL, Seidman CE, Seidman JG. Mol Cell Biol. 2001 Mar; 21(5):1730-6.
Cardiac expression of the ventricle-specific homeobox gene Irx4 is modulated by Nkx2-5 and dHand. Bruneau BG, Bao ZZ, Tanaka M, Schott JJ, Izumo S, Cepko CL, Seidman JG, Seidman CE. Dev Biol. 2000 Jan 15; 217(2):266-77.
Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome. Bruneau BG, Logan M, Davis N, Levi T, Tabin CJ, Seidman JG, Seidman CE. Dev Biol. 1999 Jul 01; 211(1):100-8.
Characterization of natriuretic peptide production by adult heart atria. Ogawa T, Vatta M, Bruneau BG, de Bold AJ. Am J Physiol. 1999 06; 276(6):H1977-86.
Regulation of chamber-specific gene expression in the developing heart by Irx4. Bao ZZ, Bruneau BG, Seidman JG, Seidman CE, Cepko CL. Science. 1999 Feb 19; 283(5405):1161-4.
BNP gene expression is specifically modulated by stretch and ET-1 in a new model of isolated rat atria. Bruneau BG, Piazza LA, de Bold AJ. Am J Physiol. 1997 12; 273(6):H2678-86.
Tissue-specific regulation of renal and cardiac atrial natriuretic factor gene expression in deoxycorticosterone acetate-salt rats. Ogawa T, Bruneau BG, Yokota N, de Bold ML, de Bold AJ. Hypertension. 1997 Dec; 30(6):1342-7.
Mutations in human TBX3 alter limb, apocrine and genital development in ulnar-mammary syndrome. Bamshad M, Lin RC, Law DJ, Watkins WC, Krakowiak PA, Moore ME, Franceschini P, Lala R, Holmes LB, Gebuhr TC, Bruneau BG, Schinzel A, Seidman JG, Seidman CE, Jorde LB. Nat Genet. 1997 Jul; 16(3):311-5.
Evidence for load-dependent and load-independent determinants of cardiac natriuretic peptide production. Ogawa T, Linz W, Stevenson M, Bruneau BG, Kuroski de Bold ML, Chen JH, Eid H, Schölkens BA, de Bold AJ. Circulation. 1996 Jun 01; 93(11):2059-67.
Alpha 1-adrenergic stimulation of isolated rat atria results in discoordinate increases in natriuretic peptide secretion and gene expression and enhances Egr-1 and c-Myc expression. Bruneau BG, Piazza LA, de Bold AJ. Endocrinology. 1996 Jan; 137(1):137-43.
Dissociation of cardiac hypertrophy, myosin heavy chain isoform expression, and natriuretic peptide production in DOCA-salt rats. Yokota N, Bruneau BG, Fernandez BE, de Bold ML, Piazza LA, Eid H, de Bold AJ. Am J Hypertens. 1995 Mar; 8(3):301-10.
Atrial natriuretic factor significantly contributes to the mineralocorticoid escape phenomenon. Evidence for a guanylate cyclase-mediated pathway. Yokota N, Bruneau BG, Kuroski de Bold ML, de Bold AJ. J Clin Invest. 1994 Nov; 94(5):1938-46.
Selective changes in natriuretic peptide and early response gene expression in isolated rat atria following stimulation by stretch or endothelin-1. Bruneau BG, de Bold AJ. Cardiovasc Res. 1994 Oct; 28(10):1519-25.