Our core facilities and highly trained staff provide state-of-the-art services to scientists at Gladstone, UC San Francisco, and the Bay Area research community.
Assay Development and Drug Discovery
The Assay Development and Drug Discovery Core helps scientists establish new assays or adapt existing ones for high-throughput screening. Their expertise accelerates research into target and drug-discovery phases and moves findings into clinical trials. The core complements high-throughput facilities for large small-molecule libraries, and supports screens for novel small-molecule leads that might become the basis of new drugs.
- Development and validation of high-throughput assays
- Transfer of assays to high-throughput screening facilities
- Fully annotated library of bioactive small molecules for drug discovery
- Screening of small focused libraries
- Contact-to-contract research organizations and consultants for medicinal chemistry
Anke Meyer-Franke, PhD
The Behavioral Core supports a wide range of projects investigating complex behavioral functions related to neurological and psychiatric diseases. The modulation of behavioral outcome measures can provide important insights into clinically relevant disease processes, as well as into basic functions of the nervous system. Our expert team looks forward to helping you unravel meaningful neurobiological pathways, validate novel therapeutic targets, and assess the efficacy of promising new treatment strategies. The core provides advanced equipment and expertise across a wide range of behavioral assessments within the following domains, all customizable to fit your exact behavioral needs.
- Neurological screens and reflexive behaviors
- Anxiety/Depression-related tests
- Sensorimotor tests
- Social/Emotional/Aggression-related tests
- Cognitive tests
- Custom designs
Jeff Simms, MA
The Bioinformatics Core provides expertise on experimental design and data analysis of complex data sets, with a specialization on analysis of large-scale data sets acquired from various cutting-edge technologies. Make the core part of your team for experimental design, power analysis, sequence analysis, annotation, pathway and network analysis, visualization, data integration, figure generation, and technical writing. It is dedicated to training, offering hands-on workshops covering fundamentals and new techniques in bioinformatics, statistics, and data science. The core also conducts its own research and technology development program, creating software tools and resources adopted by researchers worldwide.
- Consultation on various topics, including experimental design, power analysis, bioinformatics, and statistical analysis
- Collaboration, grant writing, and publications
- Bulk RNA-seq, single-cell RNA-seq, ChIP-seq, ATAC-seq, and other epigenetics analysis
- Integrated analysis including various data modalities
- Network and pathway analysis, modeling, and data visualization
- Training and workshops on topics in bioinformatics, statistics, and data science
Bioinformatics Core Staff
The Flow Cytometry Core provides flow cytometry and cell-sorting services, giving researchers a range of experimental options. The core also offers assistance with experiment design, data analysis, advice on specific applications, and technical guidance for users.
- Sorting and analysis of samples with blood-borne pathogens
- Sorting (up to 17 parameters 10 colors) and analysis (currently up to 20 parameters 12 colors)
- High Throughput Sampler capabilities for multiparametric flow cytometry
- Calcium flux, mitochondrial, and other functional assays
- DNA analysis and stem cell side population sorting
- Single cell sorting for cloning and sequencing applications (cloning)
- Sorting up to four populations at once
The Genomics Core provides state-of-the-art genomic and sequencing services to support basic and translational medical research for academic institutions and industry sectors. We strive to enable access to cost-effective, customized genomic analyses, as a functional extension of your lab. The core operates in close interactions with other Gladstone facilities, UC San Francisco core labs, and 10x Genomics. Our team actively reviews the latest genomic techniques and incorporates them to our platforms.
- 10x Genomics Chromium single cell/nucleus solutions
- Visium spatial transcriptomics for FFPE and fresh-frozen tissues
- Customized NGS library preparation based on project goals
- Illumina sequencing through the Gladstone Genomics Core and UCSF CAT
- Sample preparation and QC checks for library preparation
- Bioanalyzer, Covaris S2, and Qubit available for use by researchers
- Consultations on genomics, epigenomics and transcriptomics projects
Horng-Ru Lin, PhD
Histology and Light Microscopy
The Histology and Light Microscopy Core provides technical assistance, training, consultation, and collaboration with all aspects of experimental design, sample preparation, image acquisition, processing, and quantitative data analysis. It is equipped with state-of-the-art technologies and expertise in histology, high-resolution histological imaging, confocal, light-sheet, spinning disk microscopy, optical projection tomography, tissue clearing, and RNAscope.
- Histology: tissue dissection, fixation, paraffin processing, and embedding, and frozen (cryo) processing, sectioning, and staining (H&E, IF, IHC, and others)
- Advanced Microscopy: confocal, spinning disk, light sheet, super-resolution, whole-slide scanning, and widefield microscopy
- Quantitative Data and Image Analysis (using Imaris, Volocity, ImageJ/FIJI, MATLAB)
- Training, consulting, and collaborations
Blaise Ndjamen, PhD
The Mass Spectrometry technology center provides investigators with state-of-the-art technology to characterize protein dynamics in biological systems. Comprising five mass spectrometry systems, the center provides a number of proteomics approaches, including characterization of protein-protein interactions, composition analysis of complex protein mixtures, quantitative analysis of protein abundance, and characterization of post-translational modifications.
- Affinity purification/immunoprecipitation to identify protein-protein interactions
- Identification of post-translational modifications on purified proteins
- Global studies (protein abundance or post-translational modifications)
- Seminars and workshops on mass spectrometry methods
Danielle Swaney, PhD
The Stem Cell Core provides the physical space, all equipment, and reagents for new or established researchers to perform their stem cell–related research. They provide comprehensive human induced pluripotent stem cells–related scientific training and services, and offer potential collaborations for custom requests related to stem cell research. In addition, the core has a select number of wildtype and CRISPR-modified induced pluripotent stem (iPS) cell lines for purchase.
- Hands-on training for human iPS cell culture, iPS cell reprogramming, cardiac differentiation
- Mycoplasma screening
- Cell banking of human iPS cells
- Human iPS cell reprogramming from blood or fibroblasts
- Human iPS cell lineage differentiations (e.g., cardiac)
- Custom CRISPR-mediated gene knockouts in human iPS cell lines
Stem Cell Core Staff
Transgenic Gene Targeting
The Transgenic and Gene Targeting Core has been an integral part of Gladstone’s research for more than 20 years, producing mouse models as a service to investigators. Transgenic and knockout technologies enable scientists to learn how specific genes and proteins, or their variants, function in a natural living animal environment with a defined genetic background.
- DNA microinjection
- ES Cell microinjection
- CRISPR-mediated knock-out and knock-in
Junli Zhang, MD