1. SCIENCE
  2. CORE FACILITIES
  3. Genomics

Genomics

The Genomics Core provides state-of-the-art genomic and sequencing services to support basic and translational medical research for academic institutions and industry sectors. We strive to enable access to cost-effective, customized genomic analyses, as a functional extension of your lab.

The core closely collaborates with other Gladstone core facilities, UC San Francisco Center for Advanced Technology, UC San Francisco Core Immunology Lab, and 10x Genomics. Our approach of providing consultation prior to experiment design as well as incorporating the latest techniques, makes our services a crucial resource for all researchers.

Capabilities

Consultation

Consultation

We provide consultations on experiment design, sample preparation, experimentation, and data interpretation. We involve 10X Genomics and the Bioinformatics Core in project discussions if necessary.

10x Genomics Single Cell Solutions

10x Genomics Single Cell Solutions

We provide assays to profile gene expression and open chromatin regions at the single cell level. Our services include cell/nucleus partitioning through library construction to sequencing with all necessary QC checks.

Visium Spatial Transcriptomics

Visium Spatial Transcriptomics

Spatial transcriptomics is a next-generation molecular profiling solution that allows the researcher to gain a holistic understanding of gene expression in the tissue microenvironment. Visium projects are processed as a joint collaboration between the Histology and Light Microscopy Core and Genomics Core.
Library Construction and Sequencing

Library Construction and Sequencing

 Our core is experienced in constructing libraries from a diverse set of biological systems using varied methods. We offer customized library preparation based on your project goals. Sequencing on the Illumina platforms is carried out at Gladstone and UC San Francisco CAT.

Services

  • 10x Genomics Chromium single cell/nucleus solutions: 3’/5’ Gene Expression, V(D)J Profiling, ATAC and Multiome (ATAC + Gene Expression)
  • Visium spatial transcriptomics for FFPE and fresh-frozen tissues
  • Customized NGS library preparation: DNA/RNA-seq, SMART-seq, Amplicon seq, PacBio SMRT seq, CRISPR-based single-cell screens, etc.
  • Sequencing on the Illumina platforms at the Gladstone Genomics Core and UC San Francisco CAT
  • Sample preparation and QC: nucleic acid extraction, DNA/chromatin shearing, Bioanalyzer analysis, nucleic acid quantitation by Qubit and qPCR
  • Bioanalyzer, Covaris S2, Qubit and Nanodrop available for use by researchers
  • Consultations on genomics, epigenomics, and transcriptomics projects

Equipment

  • Visium CytAssist

  • Countess 3 FL Automated Cell Counter

  • 2100 Bioanalyzer

  • NanoDrop One Spectrophotometer

  • Qubit 2.0 Fluorometer

  • S2 Focused-Ultrasonicator

  • Illumina NextSeq2000

Fees & Scheduling

You can view our services and fees in iLab.

Scheduling

You can access these instruments with prior training from Genomics Core staff.

    • Agilent 2100 Bioanalyzer
    • Covaris S2 Focused-Ultrasonicator
    • Invitrogen Qubit 2.0 Fluorometer
    • Illumina NextSeq2000

To schedule time with one of these instruments, visit the iLab Equipment Calendar.

Contact us to schedule equipment training, schedule a consultation, or to arrange sample drop offs.

FAQs

To submit samples, register and submit a request in our online system, iLab. Once we receive and approve your request, we will contact you to coordinate sample drop off.

You can find our sample submission requirements in iLab.

Turnaround times may vary, depending on the queue at the time of submission but this is our general timeline.

  • RNA extraction, Bioanalyzer, Quantitation: 1 Week
  • Library Preparation: 2–3 Weeks
  • Sequencing (NextSeq 2000): 1 Week
  • For NovaSeq sequencing, the turnaround time will vary depending on the queue at UCSF CAT.

In publications, we ask that you include an acknowledgment to the Gladstone Genomics Core, e.g., We thank [NAME] of the Gladstone Genomics Core for their assistance with [ASSAY/PROJECT].

In addition, send us an email to let us know when your Gladstone-supported work is published

Publications

Contact

Mylinh Bernardi
Assistant Core Director
Email

Core Members

Felicia Miller, MS
Research Technologist III

Jane Srivastava
Scientific Resource Director

Events

The Genomics Core hosts a number of events from training to product demos.

View Upcoming Events

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