1. SCIENCE
  2. CORE FACILITIES
  3. Stem Cell

Stem Cell

The Stem Cell Core offers a fully functional tissue culture facility with specialized instruments, media, reagents, cell lines, and expertise for new or established researchers to perform cutting-edge stem cell research. In addition, our core provides comprehensive human induced pluripotent stem (iPS) cells–related training, consultations, and standard and customized scientific services, including derivation of iPS cells, and differentiation of iPS cells, to stem cell researchers.

Services

The Stem Cell Core specializes in the generation, maintenance and differentiation of human iPS cells. The core has expertise in human iPS cell reprogramming and iPS cell differentiation. The core supports researchers by providing the physical space, validated reagents, iPS cell lines and hands-on training for scientists to use iPS cells for their research goals.

  • iPS Cell Reprogramming from Human Peripheral Blood Mononuclear Cells (PBMCs) 
  • iPS Cell Reprogramming from Human Fibroblasts 
  • Peripheral Blood Mononuclear Cell (PBMC) Isolation from Whole Human Blood
  • Modulating Wnt Signaling
  • StemCell Technologies Kit
  • Human iPS Cell Culture and Maintenance
  • Cardiomyocyte Differentiation from Human iPS Cells
  • Human iPS Cell Reprogramming from Fibroblasts and PBMCs
  • iPSC Colony Picking
  • WTC-iPSC
  • WTC NGN2 iPSC
  • WTB-iPSC
  • CRISPRi-iPSC
  • CRISPRn-iPSC
  • GCaMP6f-iPSC
  • WTC11
  • WTC10
  • WTB
  • CRISPRi Gen1C
  • CRISPRi Gen2C
  • CRISPRi Gen1B
  • CRISPRn Gen1C 
  • WTC11-GCaMP6f
  • WTC NGN2

Visit the Conklin Lab website for more information.

  • Human iPS Cells
  • Human Skin Fibroblasts
  • “Clean up” of iPS Cells with Significant Spontaneous Differentiation for an Additional Fee
  • Mycoplasma Screening
  • Pluripotency Evaluation by Trilineage Differentiation and Fluorescent ICC Staining

Related Center

A researcher in a blue lab coat and teal gloves examines a small clear sample vial held up to the light in a laboratory setting.

Gladstone-CIRM Shared Resources Laboratory

The Stem Cell Core partners with the Gladstone-CIRM Shared Resources Laboratory to offer a streamlined platform for stem cell-based disease modeling, drug screening, and CRISPR screening for therapeutic development.
Learn more →

Equipment

Echo Revolve Microscope

Echo Revolve Microscope

Specifications

  • Hybrid microscope for upright and inverted observation methods
  • 5 MP monochrome camera (upgraded from 3.2 MP) for enhanced field of view and fluorescence imaging resolution
  • 5 MP CMOS color camera for brightfield imaging
  • Intelligent objective nosepiece for automatic recognition of the objective being used
  • iPad® tablet with retina display replaces traditional eyepieces for easier viewing
  • Supports transmitted light, phase contrast, darkfield, polarized light, and fluorescence in both upright and inverted configurations
  • Detects DAPI, FITC, TRITC Rhodamine and Texas Red

Manufacturer Site

Lonza 4D-Nucleofector Y Unit

Lonza 4D-Nucleofector Y Unit

Applications:

  • Nucleofecting adhered cells in 24-well format
  • Nucleofecting of cells at any time point during this culture period, i.e. at a later developmental stage

Specifications:

  • Disposable conductive polymer Dipping Electrode Arrays are inserted into standard 24-well culture plates for Nucleofection
  • Compatible with many cell types including primary cells
  • Range of nucleofection conditions
Invitrogen EVOS M5000 Imaging System

Invitrogen EVOS M5000 Imaging System

Specifications

  • A fully integrated digital inverted microscope
  • Designed for 3-color fluorescence (BFP, GFP, RFP), transmitted-light, and color imaging
  • Interchangeable optics, autofocus, and single-click multi-channel acquisition
  • Offers post-capture tools such as brightness/contrast adjustments, annotations, and automated cell counting
  • Includes autofocus, Z-stack capability and multi-channel capture
  • On-board software for image acquisition, analysis, and cell counting

Manufacturer Site

EVOS FL Core Imaging System

EVOS FL Core Imaging System

The EVOS FL is a comprehensive, high-performance fluorescence microscope crafted for both efficiency and user-friendliness. Its integrated onboard computer and imaging software enables users to directly capture, manage, and store multichannel fluorescence images and data from the microscope. This microscope is housed within a biosafety cabinet, making it well-suited for the picking of iPS cell/ES colonies.

Manufacturer Site

Maestro Edge

Maestro Edge

Maestro Edge MEA and Impedance system is ideal for low-throughput experiments. Record from 6- and 24-well MEA plates, plus 96-well impedance plates. Explore cell functionality easily in multiwell setups, whether tracking electrical activity or cell growth.

Manufacturer Site

Fees and Scheduling

  • Use of the Stem Cell Core facility is subject to fees charged as an add-on cost to in-house media purchased; alternatively, fees are charged via the purchase of a “sticker”. The add-on cost covers the use of plastics, PPE, some commonly used reagents, and standard equipment usage.
  • Costs/fees for media, reagents, scientific services, and specialized equipment usage are listed on iLab. An iLab account is required to view specific pricing.
  • The first 30-minute consultation is free of charge.
  • For all fee inquiries or to set up an iLab account, email stemcell-core@gladstone.ucsf.edu.
  • For custom scientific project inquiries or inquiries about use of specialized instruments, email stemcell-core@gladstone.ucsf.edu.
  • To schedule new consultations and service requests, contact stemcell-core@gladstone.ucsf.edu.
  • To schedule equipment use, visit iLab and book in the calendar.

FAQs

Contact us at stemcell-core@gladstone.ucsf.edu.

All non-Gladstone investigators need a service contract for the services provided by the Stem Cell Core.

Consumables, services, and instrument usage are paid via iLab. We would set up an iLab account for you and you would submit a fund number (PO number) to order through your account.

Yes, email stemcell-core@gladstone.ucsf.edu to discuss your project.

It depends on the project request and current workload of the Stem Cell Core. We generally require at least 2 weeks’ notice to schedule new projects. Contact the core early to schedule your project.

You may be eligible for Gladstone badge access, which will give you access to the facility during off-peak times.

All non-Gladstone investigators require a Material Transfer Agreement for iPS cell lines provided by the Stem Cell Core.

Publications

Core Members

Sam Hu
Research Technologist II

Wendy Runyon
Research Scientist

Jane Srivastava
Scientific Resource Director

Contact

Email

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