Our core facilities and highly trained staff provide state-of-the-art services to scientists at Gladstone, UC San Francisco, and the Bay Area research community. 

Assay Development Core

 

Assay Development and Drug Discovery

The Assay Development and Drug Discovery Core helps scientists establish new assays or adapt existing ones for high-throughput screening. Their expertise accelerates research into target and drug-discovery phases and moves findings into clinical trials. The core complements high-throughput facilities for large small-molecule libraries, and supports screens for novel small-molecule leads that might become the basis of new drugs.

Services Provided

  • Development and validation of high-throughput assays
  • Transfer of assays to high-throughput screening facilities
  • Fully annotated library of bioactive small molecules for drug discovery
  • Screening of small focused libraries
  • Contact-to-contract research organizations and consultants for medicinal chemistry

Contact

Anke Meyer-Franke, PhD
Staff Research Scientist III

Behavioral

The Behavioral Core supports a wide range of projects investigating complex behavioral functions related to neurological and psychiatric diseases. The modulation of behavioral outcome measures can provide important insights into clinically relevant disease processes, as well as into basic functions of the nervous system. Our expert team looks forward to helping you unravel meaningful neurobiological pathways, validate novel therapeutic targets, and assess the efficacy of promising new treatment strategies. The core provides advanced equipment and expertise across a wide range of behavioral assessments within the following domains, all customizable to fit your exact behavioral needs.

Services Provided

  • Neurological screens and reflexive behaviors
  • Anxiety/Depression-related tests
  • Sensorimotor tests
  • Social/Emotional/Aggression-related tests
  • Cognitive tests
  • Custom designs

Contact

Jeff Simms, MA
Research Scientist

Bioinformatics

The Bioinformatics Core provides expertise on experimental design and data analysis of complex data sets, with a specialization on analysis of large-scale data sets acquired from various cutting-edge technologies. Make the core part of your team for experimental design, power analysis, sequence analysis, annotation, pathway and network analysis, visualization, data integration, figure generation, and technical writing. It is dedicated to training, offering hands-on workshops covering fundamentals and new techniques in bioinformatics, statistics, and data science. The core also conducts its own research and technology development program, creating software tools and resources adopted by researchers worldwide.

Services Provided

  • Consultation on various topics, including experimental design, power analysis, bioinformatics, and statistical analysis
  • Collaboration, grant writing, and publications
  • Bulk RNA-seq, single-cell RNA-seq, ChIP-seq, ATAC-seq, and other epigenetics analysis
  • Integrated analysis including various data modalities
  • Network and pathway analysis, modeling, and data visualization
  • Training and workshops on topics in bioinformatics, statistics, and data science

Contact

Alex Pico, PhD
Senior Staff Research Scientist

Flow Cytometry

The Flow Cytometry Core provides flow cytometry and cell-sorting services, giving researchers a range of experimental options. The core also offers assistance with experiment design, data analysis, advice on specific applications, and technical guidance for users.

Services Provided

  • Sorting and analysis of samples with blood-borne pathogens
  • Sorting (up to 17 parameters 10 colors) and analysis (currently up to 20 parameters 12 colors)
  • High Throughput Sampler capabilities for multiparametric flow cytometry
  • Calcium flux measurements
  • DNA analysis and stem cell side population sorting
  • Single cell sorting for cloning and sequencing applications (cloning)
  • Sorting up to four populations at once

Contact

Genomics

The Genomics Core offers next-generation sequencing services (NGS) to enable cutting-edge genomics, transcriptomics, and epigenomics studies. It offers several types of library preparations for NGS, including quality control steps and sequencing on Illumina platforms. The core continually expands its range of services with the latest techniques.

Services Provided

  • Sequencing on IlluminaNextSeq 500 and MiSeq
  • Single-cell RNA Seq
  • Library preparation for RNA-seq, small RNA-seq, ChIP-seq, and others
  • Quality control and sample preparation
  • Bioanalyzer, Covaris S2, Qubit, and Nanodrop available for use by researchers

Contact

Jim McGuire
Research Scientist

Histology and Light Microscopy

The Histology and Light Microscopy Core provides technical assistance, training, consultation, and collaboration with all aspects of experimental design, sample preparation, image acquisition, processing, and quantitative data analysis. It is equipped with state-of-the-art technologies and expertise in histology, high-resolution histological imaging, confocal, light-sheet, spinning disk microscopy, optical projection tomography, tissue clearing, and RNAscope.

Services Provided

  • Histology: tissue dissection, fixation, paraffin processing, and embedding, and frozen (cryo) processing, sectioning, and staining (H&E, IF, IHC, and others) 
  • Advanced Microscopy: confocal, spinning disk, light sheet, super-resolution, whole-slide scanning, and widefield microscopy
  • Quantitative Data and Image Analysis (using Imaris, Volocity, ImageJ/FIJI, MATLAB)
  • Training, consulting, and collaborations

Contact

Blaise Ndjamen, PhD
Staff Research Scientist II

Mass Spectrometry

The Mass Spectrometry technology center provides investigators with state-of-the-art technology to characterize protein dynamics in biological systems. Comprising five mass spectrometry systems, the center provides a number of proteomics approaches, including characterization of protein-protein interactions, composition analysis of complex protein mixtures, quantitative analysis of protein abundance, and characterization of post-translational modifications.

Services Provided

  • Affinity purification/immunoprecipitation to identify protein-protein interactions
  • Identification of post-translational modifications on purified proteins
  • Global studies (protein abundance or post-translational modifications)
  • Seminars and workshops on mass spectrometry methods

Contact

Danielle Swaney, PhD
Visiting Scientist

Stem Cell

The Stem Cell Core provides the physical space, all equipment, and reagents for new or established researchers to perform their stem cell–related research. They provide comprehensive human induced pluripotent stem cells–related scientific training and services, and offer potential collaborations for custom requests related to stem cell research. In addition, the core has a select number of wildtype and CRISPR-modified induced pluripotent stem (iPS) cell lines for purchase.

Services Provided

  • Hands-on training for human iPS cell culture, iPS cell reprogramming, cardiac differentiation
  • Mycoplasma screening
  • Cell banking of human iPS cells
  • Human iPS cell reprogramming from blood or fibroblasts
  • Human iPS cell lineage differentiations (e.g., cardiac)
  • Custom CRISPR-mediated gene knockouts in human iPS cell lines

Contact

Po-Lin So, PhD
Senior Staff Research Scientist

Transgenic Gene Targeting

The Transgenic and Gene Targeting Core has been an integral part of Gladstone’s research for more than 20 years, producing mouse models as a service to investigators. Transgenic and knockout technologies enable scientists to learn how specific genes and proteins, or their variants, function in a natural living animal environment with a defined genetic background.

Services Provided

  • DNA microinjection
  • ES Cell microinjection
  • CRISPR-mediated knock-out and knock-in
  • TARGATT site-specific transgenic service

Contact

Junli Zhang, MD
Staff Research Scientist II