Melanie Ott’s lab studies viruses that infect humans, and applies the lessons learned to new and emerging viruses. Founded at the peak of the AIDS epidemic, the lab has since broadened its scope from HIV to other viruses with global relevance such as hepatitis C virus, Zika virus, and SARS-CoV-2. The Ott Lab combines broad expertise—in virology, cell biology, biochemistry, systems biology, and chromatin biology—with a diverse and highly collaborative approach. They focus on human-host factors restricting or enabling viral infections, and build and study complex primary cell systems, such as organoids, to model physiological conditions closely. Ott’s team leads the HOPE Collaboratory, an NIH-funded multidisciplinary research consortium dedicated to eradicating HIV. The Ott lab also leads projects on respiratory virus infections in NIH-funded UCSF QCRG AViDD and HPMI programs.

Disease Areas

COVID-19/SARS-CoV-2
HIV/AIDS
Zika
Influenza
Hepatitis
Bunya

Areas of Expertise

Virology
Chromatin Biology
Organoids
Lipid Droplets
CRISPR Diagnostics
Reverse Genetics
Working in the Ott lab

Lab Focus

Developing a diagnostic platform that combines CRISPR-based biochemistry with smartphone optics for the rapid and quantitative detection of RNA viruses—including SARS-CoV-2 and HIV.
Utilizing human organoids derived from adult tissues, adult stem cells, or induced pluripotent stem cells to model infection of the lung, liver, intestine, brain, or airways.
Building a library of all the proteins encoded in the genomes of HIV, Zika, influenza, SARS-CoV-2, and others as a resource for cross-cutting virology research.
Adapting single-cell and bulk genomic, transcriptomic, and proteomic technology to analyze organoids and immune cells, and innovative chromatin tools to study latent HIV provirus.
Developing reverse genetics tools for SARS-CoV-2 (infectious clones, replicons, and virus-like particles) to study viral variants, the effect of mutations on viral fitness, and screen antiviral compounds rapidly.

Research Impact

The Ott Lab has uncovered a number of ways in which viruses harness the biology of human cells to their own benefit and to the detriment of their hosts. 

These findings pave the way for the development of therapies that target the virus-host interface.

For instance, the lab demonstrated the importance of non-histone protein acetylation in HIV transcription and latency, ushering in the use of drugs that block acetylation as a potential step toward the eradication of HIV. They discovered a major pathway explaining hepatitis C virus’s dependence on lipid droplets inside liver cells, and identified nonsense-mediated RNA decay as a new host defense against RNA viruses that is inactivated by Zika virus.

Since the beginning of the COVID-19 pandemic, Ott’s lab and collaborators developed a testing platform that detect SARS-CoV-2 RNA without amplification, using CRISPR/Cas13a and a mobile phone. The platform could be deployed for rapid molecular testing at home or at a point-of-care facility.

They have used genome-wide CRISPR screens to identify host pathways enabling the replication of SARS-CoV-2 and of common cold coronaviruses as the basis for new pan-coronaviral therapeutic strategies.

They have also characterized Delta, Epsilon, and Omicron variants with pseudotyped viruses, virus-like particles and full-length molecular clones to identify mutations in S and N proteins that increase viral spread.

 

Professional Titles

Senior Investigator and Director, Gladstone Institute of Virology

Director, Gladstone Institute of Virology

Senior Investigator, Gladstone Institutes

Senior Vice President, Gladstone Institutes

Professor, Department of Medicine, UC San Francisco

Director, Michael Hulton Center for HIV Cure Research

Bio

In Melanie Ott’s lab, the scientific goal is clear: We research the viruses of today to prepare for the viruses of tomorrow. Toward that end, she and her team are unraveling how viruses—including hepatitis C, HIV, SARS-CoV-2, and Zika—hijack human cells, and they are designing ways to thwart these pathogens.

A native of Germany, earned her medical degree in 1991 from the University of Frankfurt/Main. During her neurology residency in Frankfurt, she watched an overwhelming number of people with HIV die for lack of a treatment or cure. Seeking answers, Ott moved to the U.S. to study HIV in the laboratory and earned her PhD from the Elmezzi Graduate School of Molecular Medicine at Northwell Health. Following a five-year stint leading her own lab at the German Cancer Research Center, and working closely with virologist and Nobel laureate Harald zur Hausen, Ott returned to the U.S. in 2002 to join Gladstone Institutes.

Here, in addition to her laboratory investigations, Ott directs the global HOPE (HIV Obstruction by Programming Epigenetics) Collaboratory, which seeks to both silence and permanently remove HIV from the body. She also lends her scientific expertise to the council of the National Institute of Drug Abuse (NIDA) and to the FDA’s Cellular, Tissue, and Gene Therapies Advisory Committee. For InvisiShield Technologies Ltd, a company developing therapeutics to prevent respiratory infections, she chairs the scientific advisory board.

Always a proponent of opening laboratory doors to any student interested in science, Ott founded the PUMAS (Promoting Underrepresented Minorities Advancing in the Sciences) summer internship program at Gladstone, for which she has received much recognition.

What are you most excited about in your lab right now?

“I am truly excited about the incredible motivation and dedication of my lab, the sense that our work is making a difference in people’s lives and the speed with which we can now progress from viral genotype to disease phenotype. Our rapid reverse genetics technology allows us to safely study any viral variant, its replication capacity and ability to cause disease in less than 2 weeks, and is widely shared with our colleagues.”

Melanie Ott, MD, PhD

Honors and Awards

2020 Named one of the Most Influential Women in the Bay Area by "San Francisco Business Times"

2020 Inspire Award, "San Francisco Business Times"

2019 NIH Merit Award, National Institutes of Health

2018 UCSF AIDS Research Institute Award for Outstanding Teaching and Mentoring

2018 Elected fellow, American Academy of Microbiology

2017 Pantheon Award: Biotechnology Educator of the Year, California Life Sciences Association

2014 Avant-Garde Award, National Institutes of Health

2013 Elected member, Association of American Physicians

2008 Thomas N. Burbridge Award, UC San Francisco

2008 UCSF Chancellor’s Award for Public Service, UC San Francisco

2000 Young Researcher Award, European Conference on Experimental AIDS Research, Madrid, Spain

Publications

Contact

Melanie Ott
Email
415.734.4807


Lab Members

Daniela Boehm, PhD
Staff Research Scientist II
Irene Chen, PhD
Postdoctoral Scholar
Irene Chen, PhD
Collaborator
Christian Davalos Gutierrez
Student Intern
Patricia Defechereux, PhD
Scientific Project Manager
Tyler Detomasi
Visiting Scientist
Patrik Dufault-Geleziunas
Student Intern
Daniel Fletcher, PhD
Affiliate Investigator
Parinaz Fozouni
Collaborator
Robert Furler O'Brien, PhD
Visiting Scientist
Ronnie Gascon
Research Associate II
Ludivine Grzelak, PhD
Postdoctoral Scholar
Jennifer Hayashi, PhD
Scientist
Sachin Jajoo
Research Associate I
Akshaya Jayakarunakaran
Research Associate I
Julia Kazmierski, PhD
Postdoctoral Scholar
Mir Khalid, PhD
Collaborator
Kanika Khanna, PhD
Scientific Program Leader I
Gabriella Kimmerly
Collaborator
Renuka Kumar, PhD
Scientific Program Leader II
Anna Kurianowicz
Student Intern
Quinn Langdon, PhD
Bioinformatician I
Zichong Li, PhD
Research Scientist
Katelyn Luo
Student Intern
Yusuke Matsui, MD, PhD
Scientist
Maria McCavitt-Malvido
Research Associate III
Dalul Mekonen
Student Intern
Mauricio Montano
Research Scientist
Rachel Padget, PhD
Postdoctoral Scholar
Valentina Pedrero Classen
Research Assistant
Julia Rosecrans
Research Associate I
Ursula Schulze-Gahmen, PhD
Senior Staff Research Scientist
Sukrit Silas, PhD
Visiting Investigator
Camille Simoneau, PhD
Collaborator
Ava Sorenson
Student Intern
Bharath Sreekumar, PhD
Scientist
Limeng Sun, PhD
Postdoctoral Scholar
Rahul Suryawanshi, PhD
Scientific Program Leader II
Chia-Lin Tsou, MS
Research Scientist
Reidun Twarock, PhD
Visiting Scientist
Cecilia Vagi-Szmola
Visiting Researcher
Francisco Zapatero Belinchon, PhD
Scientist