Center for Cell Circuitry Seminar
Friday, March 25, 2022

Markus Covert, PhD

Professor of Bioengineering
Stanford University

Pooled genetic libraries have improved screening throughput for mapping genotypes to phenotypes. However, selectable phenotypes are limited, restricting screening to outcomes with a low spatiotemporal resolution. Here, Markus Covert and his group integrated live-cell imaging with pooled library-based screening. To enable intracellular multiplexing, they developed a method called EPICode that uses a combination of short epitopes, which can also appear in various subcellular locations. EPICode thus enables the use of live-cell microscopy to characterize a phenotype of interest over time, including after sequential stimulatory/inhibitory manipulations, and directly connects behavior to the cellular genotype. To test EPICode’s capacity against an important milestone—engineering and optimizing dynamic, live-cell reporters—the Covert Lab developed a live-cell PKA kinase translocation reporter with improved sensitivity and specificity. The use of epitopes as fluorescent barcodes introduces a scalable strategy for high-throughput screening broadly applicable to protein engineering and drug discovery settings where image-based phenotyping is desired.

Details

Dates
March 25, 2022
Time
10:00-11:00am PDT
Location
Online
Audience
Gladstone and UCSF

The Center for Cell Circuitry Seminar connects investigators across disciplines to develop single-cell tools to map how cellular components connect into circuits. The center aims to develop new single-cell approaches that overcome limitations inherent in traditional techniques that analyze bulk populations of cells, thereby obscuring individual cell behavior.

Diversity, Equity, and Inclusion

At Gladstone, we are committed to providing events and professional development activities that resonate with our community’s diverse members. Our goal is to develop creative programming that encompasses a wide variety of ideas and perspectives to inspire, educate, and engage with everyone within our walls.

We want to effect positive change through our events and activities by providing a platform for discussions on important topics related to increasing diversity and inclusiveness in the sciences.