Matthew Spitzer, PhD, is an affiliate investigator at Gladstone Institutes. He is also an associate professor in the Departments of Otolaryngology–Head and Neck Surgery and of Microbiology and Immunology at UC San Francisco (UCSF), as well as a Werbe Endowed Professor in Head and Neck Oncology at UCSF. In addition, he is a Chan Zuckerberg Biohub Investigator and a Parker Institute for Cancer Immunotherapy Investigator.
Spitzer graduated from Georgetown University and earned a PhD in immunology at Stanford University, where he also trained as a postdoctoral scholar in cancer immunology. He joined UCSF as a Sandler Fellow and Parker Fellow in 2016, the university’s faculty in 2018, and the Gladstone-UCSF Institute of Genomic Immunology in 2022.
Spitzer focuses on the interactions between cancer and the immune system, particularly T cells and dendritic cells. He combines mechanistic studies with genome-scale investigations of immune cells using novel single-cell technology and bioinformatics tools. His work has shown that cancer alters the properties of immune cells, not only inside tumors but at distant sites as well. He is leveraging his expertise to improve cancer immunotherapies.
He is the recipient of an Early Independence Award from National Institutes of Health, a NextGen Transformative Research Award from the American Association for Cancer Research, and a Lloyd J. Old STAR Award from the Cancer Research Institute.
Publications
- Mass cytometry reveals a conserved immune trajectory of recovery in hospitalized COVID-19 patients. Burnett CE, Okholm TLH, Tenvooren I, Marquez DM, Tamaki S, Munoz Sandoval P, Willmore A, UCSF COMET Consortium, Hendrickson CM, Kangelaris KN, Langelier CR, Krummel MF, Woodruff PG, Calfee CS, Erle DJ, Ansel KM, Spitzer MH. Immunity. 2022 Jul 12; 55(7):1284-1298.e3.
- A cytotoxic-skewed immune set point predicts low neutralizing antibody levels after Zika virus infection. McCarthy EE, Odorizzi PM, Lutz E, Smullin CP, Tenvooren I, Stone M, Simmons G, Hunt PW, Feeney ME, Norris PJ, Busch MP, Spitzer MH, Rutishauser RL. Cell Rep. 2022 May 17; 39(7):110815.
- Lymph node colonization induces tumor-immune tolerance to promote distant metastasis. Reticker-Flynn NE, Zhang W, Belk JA, Basto PA, Escalante NK, Pilarowski GOW, Bejnood A, Martins MM, Kenkel JA, Linde IL, Bagchi S, Yuan R, Chang S, Spitzer MH, Carmi Y, Cheng J, Tolentino LL, Choi O, Wu N, Kong CS, Gentles AJ, Sunwoo JB, Satpathy AT, Plevritis SK, Engleman EG. Cell. 2022 05 26; 185(11):1924-1942.e23.
- Postmitotic G1 phase survivin drives mitogen-independent cell division of B lymphocytes. Singh A, Spitzer MH, Joy JP, Kaileh M, Qiu X, Nolan GP, Sen R. Proc Natl Acad Sci U S A. 2022 05 03; 119(18):e2115567119.
- Variation of Immune Cell Responses in Humans Reveals Sex-Specific Coordinated Signaling Across Cell Types. Fragiadakis GK, Bjornson-Hooper ZB, Madhireddy D, Sachs K, Chen H, McIlwain DR, Spitzer MH, Bendall SC, Nolan GP. Front Immunol. 2022; 13:867016.
- Influence of Self-MHC Class I Recognition on the Dynamics of NK Cell Responses to Cytomegalovirus Infection. Potempa M, Aguilar OA, Gonzalez-Hinojosa MDR, Tenvooren I, Marquez DM, Spitzer MH, Lanier LL. J Immunol. 2022 04 01; 208(7):1742-1754.
- A conserved immune trajectory of recovery in hospitalized COVID-19 patients. Burnett CE, Okholm TLH, Tenvooren I, Marquez DM, Tamaki S, Sandoval PM, UCSF COMET Consortium, Calfee CS, Hendrickson CM, Kangelaris KN, Langelier CR, Krummel MF, Woodruff PG, Erle DJ, Ansel KM, Spitzer MH. bioRxiv. 2022 Mar 16.
- A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human Primates. Bjornson-Hooper ZB, Fragiadakis GK, Spitzer MH, Chen H, Madhireddy D, Hu K, Lundsten K, McIlwain DR, Nolan GP. Front Immunol. 2022; 13:867015.
- Mass cytometry reveals single-cell kinetics of cytotoxic lymphocyte evolution in CMV-infected renal transplant patients. Ishiyama K, Arakawa-Hoyt J, Aguilar OA, Damm I, Towfighi P, Sigdel T, Tamaki S, Babdor J, Spitzer MH, Reed EF, Sarwal MM, Lanier LL, CMV Systems Immunobiology Group. Proc Natl Acad Sci U S A. 2022 02 22; 119(8).
- Cellular architecture of human brain metastases. Gonzalez H, Mei W, Robles I, Hagerling C, Allen BM, Hauge Okholm TL, Nanjaraj A, Verbeek T, Kalavacherla S, van Gogh M, Georgiou S, Daras M, Phillips JJ, Spitzer MH, Roose JP, Werb Z. Cell. 2022 02 17; 185(4):729-745.e20.
- Archetypes of checkpoint-responsive immunity. Im K, Combes AJ, Spitzer MH, Satpathy AT, Krummel MF. Trends Immunol. 2021 11; 42(11):960-974.
- The effect of low-dose IL-2 and Treg adoptive cell therapy in patients with type 1 diabetes. Dong S, Hiam-Galvez KJ, Mowery CT, Herold KC, Gitelman SE, Esensten JH, Liu W, Lares AP, Leinbach AS, Lee M, Nguyen V, Tamaki SJ, Tamaki W, Tamaki CM, Mehdizadeh M, Putnam AL, Spitzer MH, Ye CJ, Tang Q, Bluestone JA. JCI Insight. 2021 09 22; 6(18).
- SCITO-seq: single-cell combinatorial indexed cytometry sequencing. Hwang B, Lee DS, Tamaki W, Sun Y, Ogorodnikov A, Hartoularos GC, Winters A, Yeung BZ, Nazor KL, Song YS, Chow ED, Spitzer MH, Ye CJ. Nat Methods. 2021 08; 18(8):903-911.
- Pre-existing immune status associated with response to combination of sipuleucel-T and ipilimumab in patients with metastatic castration-resistant prostate cancer. Sinha M, Zhang L, Subudhi S, Chen B, Marquez J, Liu EV, Allaire K, Cheung A, Ng S, Nguyen C, Friedlander TW, Aggarwal R, Spitzer M, Allison JP, Small EJ, Sharma P, Fong L. J Immunother Cancer. 2021 05; 9(5).
- Systemic immunity in cancer. Hiam-Galvez KJ, Allen BM, Spitzer MH. Nat Rev Cancer. 2021 06; 21(6):345-359.
- Single-cell analysis by mass cytometry reveals metabolic states of early-activated CD8+ T cells during the primary immune response. Levine LS, Hiam-Galvez KJ, Marquez DM, Tenvooren I, Madden MZ, Contreras DC, Dahunsi DO, Irish JM, Oluwole OO, Rathmell JC, Spitzer MH. Immunity. 2021 04 13; 54(4):829-844.e5.
- Landscape of coordinated immune responses to H1N1 challenge in humans. Rahil Z, Leylek R, Schürch CM, Chen H, Bjornson-Hooper Z, Christensen SR, Gherardini PF, Bhate SS, Spitzer MH, Fragiadakis GK, Mukherjee N, Kim N, Jiang S, Yo J, Gaudilliere B, Affrime M, Bock B, Hensley SE, Idoyaga J, Aghaeepour N, Kim K, Nolan GP, McIlwain DR. J Clin Invest. 2020 11 02; 130(11):5800-5816.
- Functional CRISPR dissection of gene networks controlling human regulatory T cell identity. Schumann K, Raju SS, Lauber M, Kolb S, Shifrut E, Cortez JT, Skartsis N, Nguyen VQ, Woo JM, Roth TL, Yu R, Nguyen MLT, Simeonov DR, Nguyen DN, Targ S, Gate RE, Tang Q, Bluestone JA, Spitzer MH, Ye CJ, Marson A. Nat Immunol. 2020 11; 21(11):1456-1466.
- ImmunoGlobe: enabling systems immunology with a manually curated intercellular immune interaction network. Atallah MB, Tandon V, Hiam KJ, Boyce H, Hori M, Atallah W, Spitzer MH, Engleman E, Mallick P. BMC Bioinformatics. 2020 Aug 10; 21(1):346.
- Intratumoral CD4+ T Cells Mediate Anti-tumor Cytotoxicity in Human Bladder Cancer. Oh DY, Kwek SS, Raju SS, Li T, McCarthy E, Chow E, Aran D, Ilano A, Pai CS, Rancan C, Allaire K, Burra A, Sun Y, Spitzer MH, Mangul S, Porten S, Meng MV, Friedlander TW, Ye CJ, Fong L. Cell. 2020 06 25; 181(7):1612-1625.e13.
- Tumor Immune Profiling-Based Neoadjuvant Immunotherapy for Locally Advanced Melanoma. Levine LS, Mahuron KM, Tsai KK, Wu C, Mattis DM, Pauli ML, Oglesby A, Lee JC, Spitzer MH, Krummel MF, Algazi AP, Rosenblum MD, Alvarado M, Daud AI. Ann Surg Oncol. 2020 Oct; 27(11):4122-4130.
- Systemic dysfunction and plasticity of the immune macroenvironment in cancer models. Allen BM, Hiam KJ, Burnett CE, Venida A, DeBarge R, Tenvooren I, Marquez DM, Cho NW, Carmi Y, Spitzer MH. Nat Med. 2020 07; 26(7):1125-1134.
- Depletion of microbiome-derived molecules in the host using Clostridium genetics. Guo CJ, Allen BM, Hiam KJ, Dodd D, Van Treuren W, Higginbottom S, Nagashima K, Fischer CR, Sonnenburg JL, Spitzer MH, Fischbach MA. Science. 2019 12 13; 366(6471).
- Denisovan, modern human and mouse TNFAIP3 alleles tune A20 phosphorylation and immunity. Zammit NW, Siggs OM, Gray PE, Horikawa K, Langley DB, Walters SN, Daley SR, Loetsch C, Warren J, Yap JY, Cultrone D, Russell A, Malle EK, Villanueva JE, Cowley MJ, Gayevskiy V, Dinger ME, Brink R, Zahra D, Chaudhri G, Karupiah G, Whittle B, Roots C, Bertram E, Yamada M, Jeelall Y, Enders A, Clifton BE, Mabbitt PD, Jackson CJ, Watson SR, Jenne CN, Lanier LL, Wiltshire T, Spitzer MH, Nolan GP, Schmitz F, Aderem A, Porebski BT, Buckle AM, Abbott DW, Ziegler JB, Craig ME, Benitez-Aguirre P, Teo J, Tangye SG, King C, Wong M, Cox MP, Phung W, Tang J, Sandoval W, Wertz IE, Christ D, Goodnow CC, Grey ST. Nat Immunol. 2019 10; 20(10):1299-1310.
- De novo mutations in mitochondrial DNA of iPSCs produce immunogenic neoepitopes in mice and humans. Deuse T, Hu X, Agbor-Enoh S, Koch M, Spitzer MH, Gravina A, Alawi M, Marishta A, Peters B, Kosaloglu-Yalcin Z, Yang Y, Rajalingam R, Wang D, Nashan B, Kiefmann R, Reichenspurner H, Valantine H, Weissman IL, Schrepfer S. Nat Biotechnol. 2019 10; 37(10):1137-1144.
- Comprehensive Immune Monitoring of Clinical Trials to Advance Human Immunotherapy. Hartmann FJ, Babdor J, Gherardini PF, Amir ED, Jones K, Sahaf B, Marquez DM, Krutzik P, O'Donnell E, Sigal N, Maecker HT, Meyer E, Spitzer MH, Bendall SC. Cell Rep. 2019 07 16; 28(3):819-831.e4.
- Hacking the 'Cellular Immunology Agency': T cells caught in the act. Spitzer MH. Nat Immunol. 2019 04; 20(4):382-383.
- Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis. Hartmann FJ, Simonds EF, Vivanco N, Bruce T, Borges L, Nolan GP, Spitzer MH, Bendall SC. Methods Mol Biol. 2019; 1989:55-81.
- Autocrine TGFβ Is a Survival Factor for Monocytes and Drives Immunosuppressive Lineage Commitment. Gonzalez-Junca A, Driscoll KE, Pellicciotta I, Du S, Lo CH, Roy R, Parry R, Tenvooren I, Marquez DM, Spitzer MH, Barcellos-Hoff MH. Cancer Immunol Res. 2019 02; 7(2):306-320.
- Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry. Han G, Spitzer MH, Bendall SC, Fantl WJ, Nolan GP. Nat Protoc. 2018 10; 13(10):2121-2148.
- MetaCyto: A Tool for Automated Meta-analysis of Mass and Flow Cytometry Data. Hu Z, Jujjavarapu C, Hughey JJ, Andorf S, Lee HC, Gherardini PF, Spitzer MH, Thomas CG, Campbell J, Dunn P, Wiser J, Kidd BA, Dudley JT, Nolan GP, Bhattacharya S, Butte AJ. Cell Rep. 2018 07 31; 24(5):1377-1388.
- Engineered Tumor-Targeted T Cells Mediate Enhanced Anti-Tumor Efficacy Both Directly and through Activation of the Endogenous Immune System. Avanzi MP, Yeku O, Li X, Wijewarnasuriya DP, van Leeuwen DG, Cheung K, Park H, Purdon TJ, Daniyan AF, Spitzer MH, Brentjens RJ. Cell Rep. 2018 05 15; 23(7):2130-2141.
- Jak1 Integrates Cytokine Sensing to Regulate Hematopoietic Stem Cell Function and Stress Hematopoiesis. Kleppe M, Spitzer MH, Li S, Hill CE, Dong L, Papalexi E, De Groote S, Bowman RL, Keller M, Koppikar P, Rapaport FT, Teruya-Feldstein J, Gandara J, Mason CE, Nolan GP, Levine RL. Cell Stem Cell. 2018 02 01; 22(2):277.
- A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. Dodd D, Spitzer MH, Van Treuren W, Merrill BD, Hryckowian AJ, Higginbottom SK, Le A, Cowan TM, Nolan GP, Fischbach MA, Sonnenburg JL. Nature. 2017 11 30; 551(7682):648-652.
- Jak1 Integrates Cytokine Sensing to Regulate Hematopoietic Stem Cell Function and Stress Hematopoiesis. Kleppe M, Spitzer MH, Li S, Hill CE, Dong L, Papalexi E, De Groote S, Bowman RL, Keller M, Koppikar P, Rapaport FT, Teruya-Feldstein J, Gandara J, Mason CE, Nolan GP, Levine RL. Cell Stem Cell. 2017 10 05; 21(4):489-501.e7.
- Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells. Cavrois M, Banerjee T, Mukherjee G, Raman N, Hussien R, Rodriguez BA, Vasquez J, Spitzer MH, Lazarus NH, Jones JJ, Ochsenbauer C, McCune JM, Butcher EC, Arvin AM, Sen N, Greene WC, Roan NR. Cell Rep. 2017 07 25; 20(4):984-998.
- Systemic Immunity Is Required for Effective Cancer Immunotherapy. Spitzer MH, Carmi Y, Reticker-Flynn NE, Kwek SS, Madhireddy D, Martins MM, Gherardini PF, Prestwood TR, Chabon J, Bendall SC, Fong L, Nolan GP, Engleman EG. Cell. 2017 01 26; 168(3):487-502.e15.
- Expression of specific inflammasome gene modules stratifies older individuals into two extreme clinical and immunological states. Furman D, Chang J, Lartigue L, Bolen CR, Haddad F, Gaudilliere B, Ganio EA, Fragiadakis GK, Spitzer MH, Douchet I, Daburon S, Moreau JF, Nolan GP, Blanco P, Déchanet-Merville J, Dekker CL, Jojic V, Kuo CJ, Davis MM, Faustin B. Nat Med. 2017 Feb; 23(2):174-184.
- Akt and SHP-1 are DC-intrinsic checkpoints for tumor immunity. Carmi Y, Prestwood TR, Spitzer MH, Linde IL, Chabon J, Reticker-Flynn NE, Bhattacharya N, Zhang H, Zhang X, Basto PA, Burt BM, Alonso MN, Engleman EG. JCI Insight. 2016 11 03; 1(18):e89020.
- Mapping the Fetomaternal Peripheral Immune System at Term Pregnancy. Fragiadakis GK, Baca QJ, Gherardini PF, Ganio EA, Gaudilliere DK, Tingle M, Lancero HL, McNeil LS, Spitzer MH, Wong RJ, Shaw GM, Darmstadt GL, Sylvester KG, Winn VD, Carvalho B, Lewis DB, Stevenson DK, Nolan GP, Aghaeepour N, Angst MS, Gaudilliere BL. J Immunol. 2016 12 01; 197(11):4482-4492.
- Automated mapping of phenotype space with single-cell data. Samusik N, Good Z, Spitzer MH, Davis KL, Nolan GP. Nat Methods. 2016 06; 13(6):493-6.
- Mass Cytometry: Single Cells, Many Features. Spitzer MH, Nolan GP. Cell. 2016 May 05; 165(4):780-91.
- IMMUNOLOGY. An interactive reference framework for modeling a dynamic immune system. Spitzer MH, Gherardini PF, Fragiadakis GK, Bhattacharya N, Yuan RT, Hotson AN, Finck R, Carmi Y, Zunder ER, Fantl WJ, Bendall SC, Engleman EG, Nolan GP. Science. 2015 Jul 10; 349(6244):1259425.
- Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity. Carmi Y, Spitzer MH, Linde IL, Burt BM, Prestwood TR, Perlman N, Davidson MG, Kenkel JA, Segal E, Pusapati GV, Bhattacharya N, Engleman EG. Nature. 2015 May 07; 521(7550):99-104.
- Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm. Zunder ER, Finck R, Behbehani GK, Amir el-AD, Krishnaswamy S, Gonzalez VD, Lorang CG, Bjornson Z, Spitzer MH, Bodenmiller B, Fantl WJ, Pe'er D, Nolan GP. Nat Protoc. 2015 Feb; 10(2):316-33.
- Single-cell mass cytometry of TCR signaling: amplification of small initial differences results in low ERK activation in NOD mice. Mingueneau M, Krishnaswamy S, Spitzer MH, Bendall SC, Stone EL, Hedrick SM, Pe'er D, Mathis D, Nolan GP, Benoist C. Proc Natl Acad Sci U S A. 2014 Nov 18; 111(46):16466-71.
- Systems biology. Conditional density-based analysis of T cell signaling in single-cell data. Krishnaswamy S, Spitzer MH, Mingueneau M, Bendall SC, Litvin O, Stone E, Pe'er D, Nolan GP. Science. 2014 Nov 28; 346(6213):1250689.
- A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis. Megill A, Lee T, DiBattista AM, Song JM, Spitzer MH, Rubinshtein M, Habib LK, Capule CC, Mayer M, Turner RS, Kirkwood A, Yang J, Pak DT, Lee HK, Hoe HS. J Neurosci. 2013 May 29; 33(22):9306-18.
- The transcriptional landscape of αβ T cell differentiation. Mingueneau M, Kreslavsky T, Gray D, Heng T, Cruse R, Ericson J, Bendall S, Spitzer MH, Nolan GP, Kobayashi K, von Boehmer H, Mathis D, Benoist C, Immunological Genome Consortium, Best AJ, Knell J, Goldrath A, Joic V, Koller D, Shay T, Regev A, Cohen N, Brennan P, Brenner M, Kim F, Nageswara Rao T, Wagers A, Heng T, Ericson J, Rothamel K, Ortiz-Lopez A, Mathis D, Benoist C, Bezman NA, Sun JC, Min-Oo G, Kim CC, Lanier LL, Miller J, Brown B, Merad M, Gautier EL, Jakubzick C, Randolph GJ, Monach P, Blair DA, Dustin ML, Shinton SA, Hardy RR, Laidlaw D, Collins J, Gazit R, Rossi DJ, Malhotra N, Sylvia K, Kang J, Kreslavsky T, Fletcher A, Elpek K, Bellemare-Pelletier A, Malhotra D, Turley S. Nat Immunol. 2013 Jun; 14(6):619-32.
- Risks and benefits of testosterone therapy in older men. Spitzer M, Huang G, Basaria S, Travison TG, Bhasin S. Nat Rev Endocrinol. 2013 Jul; 9(7):414-24.
- Thyrotoxicosis with post-treatment hypothyroidism in a patient with acute suppurative thyroiditis due to porphyromonas. Spitzer M, Alexanian S, Farwell AP. Thyroid. 2012 Jan; 22(1):97-100.
- ApoE receptor 2 regulates synapse and dendritic spine formation. Dumanis SB, Cha HJ, Song JM, Trotter JH, Spitzer M, Lee JY, Weeber EJ, Turner RS, Pak DT, Rebeck GW, Hoe HS. PLoS One. 2011 Feb 15; 6(2):e17203.
- Left lateralized P300 amplitude deficit in schizophrenic patients depends on pitch disparity. Weisbrod M, Winkler S, Maier S, Hill H, Thomas C, Spitzer M. Biol Psychiatry. 1997 Mar 01; 41(5):541-9.